The 20 year old defeated World No 4, Elina Svitolina 6-3 7-6 (6) to advance to her first ever Wimbledon quarterfinals.
Slovakia's Magdalena Rybarikova advanced to her first Grand Slam quarterfinal after a 6-4, 2-6, 6-3 win over Croatian qualifier Petra Martic.
10 seed, made her debut in the grasscourt major back in 1997.
Serving out the first game imperiously to love, Williams moved her opponent from side to side before breaking Konjuh to go up 5-3 and closing out the set without difficulty.
Williams' straight set victory was powered by seven aces and winning 31 of 36 first-serve points in the match over Konjuh.
Again marrying winners with unforced errors - 42 played 39 respectively - Ostapenko broke her opponent six times en route to setting up a clash with Venus Williams.
That will be her 100th match at Wimbledon and few will have been more straightforward than this 6-3, 6-2 victory.
Ostapenko hasn't dropped a set in her last two matches, but was on the brink of defeat to former junior rival Francoise Abanda in the second round, outlasting the Canadian in three.
It was not easy in the third as well, with the two locked 4-4, but Paire served up yet another double-fault to allow Murray to level and break him to go up 5-4 and then close out the match 7-6 (1), 6-4, 6-4.