Justin Gatlin has regained that title again after holding off the iconic Jamaican in the 100-meter final of the World Championships of Athletics last Saturday in London. Trailing when he got the baton on the anchor leg, Bolt powered away and quickly swept past his rivals and had time to look to his right before crossing in 37.95.
Great Britain won the gold medal with the third-fastest time in history.
But there were no such problems for Bolt at London Stadium on Saturday as, following legs by Tyquendo Tracey, Julian Forte and Michael Campbell, he cruised home to send Jamaica to victory in their heat in a season's best 37.95 seconds.
Blake has not been able to train at full pace in the lead-up to the race, which will be sprint great Usain Bolt's last on the track.
On the last day of his global career, fans jostled for space to have a glimpse of Bolt at the warm-up arena, adjacent to the stadium and television cameras were fixed at him to have a shot of the towering Jamaican.
Bolt raced very lightly this year, and his best time coming into the meet was the same 9.95 seconds he ran in his finale.
It's okay though, after retiring from racing, Bolt looks nearly certain to pursue a career as a GAA player.
An 11-time gold medalist at the World Championships Bolt has always been excited when it comes to running the relays.
Bolt told the BBC post-race that, "I enjoy relays more than anything".
During Friday's competition, the most jaw-dropping result in a week of upsets came in the women's steeplechase, where the United States clinched an unlikely 1-2 finish.
They also advanced to the final. Justin Gatlin clocked in 9.92 seconds while USA's Christian Coleman registered 9.94 seconds to spoil Bolt's 9.95 seconds and his farewell party.
Provided his Jamaican team-mates escape a mistake in this morning's heats, Bolt will line up for one final swansong at 9.50pm. "It's hard to be sad because of the energy I'm getting from the crowd", said the Jamaican, who suffered a rare defeat in the 100 meters when he took bronze.
"There are no words to describe how I am feeling". I am determined to get those records and those medals back into the hands of their rightful owners.
'We have been training that relay, there were some camps.
However Britain flew out of the blocks and their ideal changeovers gave them a great chance going into the final 100m as they led both the US and Jamaica.
It was the first time Bolt had been beaten at a major championships since the 4x100m relay at the 2007 worlds in Osaka.
Jamaica clocked the third quickest time in the heats, behind the United States of America and Great Britain.
Switzerland (42.50) qualified with the fourth fastest time overall while Netherlands (42.64), Brazil (42.77) and Trinidad and Tobago (42.91) completed the final round line-up.