Around 100 officials will be involved in the counting process which will take place in 19 rounds.
Dhinakaran got 89,013 votes while Madhusudhanan polled 48,306 votes.
Instead of fighting the polls in the lanes and bylanes of RK Nagar, the DMK fought it more in court halls and chambers of the Election Commission, filing cases and e-mailing petitions.
The bypoll was necessitated after the death of former chief minister Jayalalithaa a year ago in December and is speculated to be the litmus test for the leadership of E Panneerselvam.
Dhinakaran registered a total 24,550 votes at the end of third round, continuing to maintain a comfortable margin over and above his AIADMK and DMK rivals.
Snapping at his heel was Dhinakaran, who is trying to protect the influence of the Sasikala family and destabilise the government of Chief Minister E Palanisamy.
While Political pundits are seeing TTV as a crucial leader in state after this phenomenal victory, the opposition leaders do not consider him to be major roadblock in coming general polls.
Dhinakaran, who lost the AIADMK symbol to the EPS-OPS faction, chose to contest the election as an independent candidate and was allotted a "pressure cooker" symbol for the same. However, both AIADMK and DMK have expressed confidence that they would win the seat.
The bypoll held on December 21 had seen a record 77.68% voter turnout.
RK Nagar, represented twice by Jayalalithaa, has an electorate of 2,28,234, comprising 1,10,903 men, 1,17,232 women and 99 transgender people.
The 2015 RK Nagar bypoll, Jayalalithaa was elected by a huge margin of 1.5 lakh votes.