Jacksonville have won the AFC South for the first time ever with defence and the running game.
The Bills broke the deadlock when Stephen Hauschka kicked a 31-yard field goal following a mammoth 18-play drive that lasted eight minutes and six seconds, but Jacksonville levelled the scores with a 44-yard field goal from Josh Lambo with two seconds of the first half remaining.
But three wins in four to end the season and a bit of help from Cincinnati ended the longest play-off drought in all of United States sport.
The Jaguars defense, which ranks first in the NFL in yards per play allowed (4.6), first in Football Outsiders' efficiency ratings and second in sack percentage, has been out of this world. Fournette averaged 2.7 yards per carry and also tallied 21 receiving yards on three catches.
The Jags defence intercepted Ben Roethlisberger five times, held Le'Veon Bell to 47 rushing yards and held the Steelers' high-powered offence to no touchdowns.
The Jaguars were finally able to move the ball on the final drive of the half. He was 6-of-15 passing for 33 yards. The Bills got the chance after Jaguars linebacker Telvin Smith jumped offside on fourth-and-3, giving Buffalo a first down.
Buffalo had more success (148 yards), but failed to cash in on a first-and-goal opportunity at the Jaguars' 1-yard line.
Buffalo and Jacksonville traded field goals over the final 1:49 of the second quarter and are tied 3-3 at halftime in an AFC wild-card game. It was the first career playoff game for the 11-year veteran, who signed with the Jaguars as a free agent in 2011 after being drafted by Buffalo. The Titans will take on the New England Patriots on Saturday night.